General Cell Lines and Cell Cultures
Biolegend PE anti-mouse CD24 Recombinant
PE anti-mouse CD24 Recombinant [QA20A91]; Isotype: Mouse IgG1, κ; Reactivity: Mouse; Apps: FC; Size: 100 ug
Brainxell Inc Spinal Astrocytes (Human iPSC-Derived, Male Line, 1M)
Spinal astrocytes derived from iPSC line WC-30, male. Contents: 1 vial of ≥1x10^6 viable cryopreserved cells. Marker Expression and Morphology: Spinal Astrocytes (BX-0650) express key astrocyte markers GFAP (green) at >80% and exhibit mature process-bearing morphologies (shown here with a DAPI stain). Labelling by the spinal-specific marker HOXB4 (red) with a Hoescht stain highlights the regional specificity of the Spinal Astrocytes. Function: Spinal Astrocytes promote mature motor neuron activity. Co-culturing motor neurons with spinal astrocytes promotes maximal calcium influx within 10 days. Co-culture spinal astrocytes with motor neurons also accelerates the development of activity recorded by multi-electrode array. Related diseases: Amyotrophic Lateral Sclerosis (ALS), Spinal muscular atrophy (SMA), & Spinal Cord Injury.
Eurofins Discoverx Ready-to-Assay SST5 Somatostatin Receptor Frozen Cells, 2 Vials of Cells. (HTS139RTA)
The Ready-to-Assay SST5 Somatostatin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. somatostatin) based activation of SSTR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses SSTR5 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of SSTR5, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS099RTA)
The Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ILEX LIFE SCIENCES LLC EMEM FIBROBLASTS 6 X 500 ML
PAN-Biotech EMEM Cell Culture Medium for Fibroblasts, w/o: L-Glutamine, w: 2.2 g/L NaHCO3 (6 x 500 ml). BICARBONATE: With 2.2 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: Without glutamine. PHENOL RED: With Phenol Red. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: EMEM Fibroblasts is a cell culture medium based on MEM Eagle (Eagle's Minimum Essential Medium) which was in turn developed from BME (Basal Medium Eagle). EMEM Fibroblasts is optimized specifically for the cultivation of fibroblasts. EMEM Fibroblasts is based on EMEM supplemented with additional or higher amount of amino acids and vitamins and is thus optimized for improved growth of fibroblasts. For cultivation of fibroblasts, this medium must be supplemented with 10% FBS before use.
Eurofins Discoverx PathHunter C2C12 TACR1 β-Arrestin Cell Line, 2 vials. (93-0344C7)
The PathHunter TACR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. [ligand]) based activation of TACR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TACR1 and EA-tagged β-Arrestin-2. Activation of TACR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
STEMCELL Technologies Mouse Hepatic Organoids, Size: 1 Unit
Cryopreserved Mouse Hepatic Organoids provide a convenient way to establish or standardize hepatic organoid cultures in the laboratory. Each vial contains mouse hepatic progenitor organoids (derived from the liver of a C57BL/6 mouse) that were cultured in HepatiCult™ Organoid Growth Medium (Mouse; Catalog #06030) and cryopreserved in CryoStor CS10 (Catalog #07930). Using cryopreserved Mouse Hepatic Organoids enables establishment of hepatic organoid cultures without the need to isolate hepatic ducts from primary tissue, eliminating the need for access to fresh mouse tissue. The organoids can be passaged and expanded using HepatiCult™ Organoid Growth Medium (Mouse) and refrozen in CryoStor CS10. Mouse hepatic progenitor organoid cultures can be used for research in a variety of fields, including liver biology and disease modeling, cancer, and toxicity assay development.
ATCC SK-MEL-28; Melanoma; Human (Homo sapiens)
SK-MEL-28 cells are melanocytes isolated from the skin tissue of a 51-year-old, male patient with malignant melanoma. This cell line is a suitable transfection host and has applications in cancer and toxicology research. SK-MEL-28 is one of a very extensive series of melanoma cell lines isolated by T. Takahashi and associates.
Eurofins Discoverx HEK 293 HTR2B Gq Cell Line, 2 vials. (95-1024C1)
The HTR2B Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of HTR2B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR2B, a naturally Gq coupled GPCR. Upon activation of HTR2B, the resulting calcium activity can be fluorescently assayed using the Calcium No WashPLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx InCELL Hunter U2OS Bcl2-Bax Protein Binding Cell Line, 2 vials. (96-0010C3)
The InCELL Hunter U2OS Bcl2-Bax Protein Binding Cell Line provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2-Bax binding via detection of Bax levels. This product uses EFC technology. The cell line included overexpresses Bcl2 target in an untagged form and Bax binding partner fused to ProLabel. When a compound engages the Bcl2 target protein, partner binding is disrupted and the destabilized Bax is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PrecisION hNav1.3 Recombinant Stable Cell Line, 2 vials. (CYL3003)
The PrecisION hNav1.3 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Nav1.3 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Nav1.3 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx PrecisION hNav1.4 Recombinant Stable Cell Line, 2 vials. (CYL3024)
The PrecisION hNav1.4 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Nav1.4 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Nav1.4 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx InCELL Hunter A549 BTK Kinase Binding Cell Line, 2 vials. (96-0056C15)
The InCELL Hunter A549 BTK Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of BTK via detection of BTK levels. This product uses EFC technology. The cell line included overexpresses BTK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx cAMP Hunter CHO-K1 HTR1B Gi cell Line, 2 vials. (95-0162C2)
The cAMP Hunter HTR1B Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR1B GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HTR1B, a naturally Gi/Go coupled GPCR. Upon activation of HTR1B, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.